I think about Jo’s gender a lot because it’s like

Okay obviously Jo’s uncomfortable participating in girly things, which doesn’t necessarily make Jo not a girl, but in “Runaway Model” Jo also seems discomforted by even being thought of as a girl

But Jo also doesn’t really like being referred to as a dude by Lightning, which makes me think Jo isn’t really comfortable with the idea of being either gender, so what if

Agender Jo?

Yes agender Jo